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Information

Synonym/Acronym

N/A

Rationale

To assist in evaluating increased risk for blood clots, plaque formation, and platelet aggregations associated with atherosclerosis and stroke risk.

Patient Preparation

There are no food, fluid, activity, or medication restrictions unless by medical direction.

Normal Findings

Method: Chromatography

Conventional and SI Units
HomocysteineLess than 15 micromol/L
Methylmalonic AcidLess than 0.4 micromol/L

Critical Findings and Potential Interventions

N/A

Overview

Study type: Blood collected in a gold-, red-, or red/gray-top tube if methylmalonic acid and homocysteine are to be measured together; related body system: Circulatory system. Alternatively, a lavender-top [EDTA] tube may be acceptable for the homocysteine measurement. The laboratory should be consulted before specimen collection because specimen type may be method dependent. Care must be taken to use the same type of collection container if serial measurements are to be taken.

Homocysteine is an amino acid formed from methionine. Normally, homocysteine is rapidly remetabolized in a biochemical pathway that requires vitamin B12 and folate, preventing the buildup of homocysteine in the blood. Excess levels damage the endothelial lining of blood vessels; change coagulation factor levels, increasing the risk of blood clot formation and stroke; prevent smaller arteries from dilating, increasing the risk of plaque formation; cause platelet aggregation; and cause smooth muscle cells lining the arterial wall to multiply, promoting atherosclerosis. For additional information regarding screening guidelines for atherosclerotic cardiovascular disease (ASCVD), refer to the study titled “Cholesterol, Total and Fractions.”

Approximately one-third of patients with hyperhomocystinuria have normal fasting levels. Patients with a heterozygous biochemical enzyme defect in cystathionine B synthase or with a nutritional deficiency in vitamin B6 can be identified through the administration of a methionine challenge or loading test. Specimens are collected while fasting and 2 hr later. An increase in homocysteine after 2 hr is indicative of hyperhomocystinuria. In patients with vitamin B12 deficiency, elevated levels of methylmalonic acid and homocysteine develop fairly early in the course of the disease. Unlike vitamin B12 levels, homocysteine levels will remain elevated for at least 24 hr after the start of vitamin therapy. This may be useful if vitamin therapy is inadvertently begun before specimen collection. Patients with folate deficiency, for the most part, will only develop elevated homocysteine levels. A methylmalonic acid level can differentiate between vitamin B12 and folate deficiency, since it is increased in vitamin B12 deficiency but not in folate deficiency. Hyperhomocysteinemia due to folate deficiency in pregnant women is believed to increase the risk of neural tube defects. Elevated levels of homocysteine are thought to chemically damage the exposed neural tissue of the developing fetus.

Indications

Interfering Factors

Factors That May Alter the Results of the Study

  • Drugs and other substances that may increase plasma homocysteine levels include carbamazepine, cycloserine, hydralazine, isoniazid, methotrexate, penicillamine, phenelzine, and procarbazine.
  • Drugs and other substances that may decrease plasma homocysteine levels include folic acid.

Other Considerations

  • Specimens should be kept at a refrigerated temperature and delivered immediately to the laboratory for processing.

Potential Medical Diagnosis: Clinical Significance of Results

Increased In

  • Cerebrovascular disease (there is a relationship, but the pathophysiology is unclear)
  • Chronic kidney disease (pathophysiology is unclear)
  • Coronary artery disease (CAD) (there is a relationship, but the pathophysiology is unclear)
  • Folic acid deficiency(folate is required for completion of biochemical reactions involved in homocysteine metabolism)
  • Homocystinuria (inherited disorder of methionine metabolism that results in accumulation of homocysteine)
  • Peripheral vascular disease(related to vascular wall damage and formation of occlusive plaque)
  • Vitamin B12 deficiency (vitamin B12 is required for completion of biochemical reactions involved in homocysteine metabolism)

Decreased In

N/A

Nursing Implications, Nursing Process, Clinical Judgement

Before the Study: Planning and Implementation

Teaching the Patient What to Expect

  • Discuss how this test can assist in screening for risk of cardiovascular disease and stroke.
  • Explain that a blood sample is needed for the test.
  • Obtain a history of the patient’s health concerns (symptoms, surgical procedures) and results of previous laboratory and diagnostic studies.

Potential Nursing Actions

  • Investigate for a family history of heart disease, smoking, obesity, poor diet, lack of physical activity, hypertension, diabetes, previous myocardial infarction, and previous vascular disease.
  • Explain that knowledge of genetics assists in identifying those who may benefit from additional education, risk assessment, and counseling.
  • Discuss how evaluation of genetic factors can provide direction for risk assessment related to development of type 2 diabetes, CAD, myocardial infarction, or ischemic stroke.

After the Study: Implementation & Evaluation Potential Nursing Actions

Treatment Considerations

  • Monitor and trend homocysteine levels as an increase may be associated with atherosclerosis or CAD. Individuals who have specific risk factors and/or existing medical conditions (e.g., elevated low-density lipoprotein cholesterol levels, other lipid disorders, type 1 diabetes, type 2 diabetes, insulin resistance, or metabolic syndrome) may also demonstrate increased homocysteine levels.
  • A variety of dietary patterns are beneficial for people with ASCVD. For additional information regarding nutritional guidelines, refer to the study titled “Cholesterol, Total and Fractions.”
  • Modifying lifestyle choices can decrease risk, including safely decrease sodium intake, achieve a normal weight, manage blood pressure, ensure regular participation of moderate aerobic physical activity three to four times per week, eliminate tobacco use, and adhere to a heart-healthy diet.
  • Advise those with elevated triglycerides to eliminate or reduce alcohol.

Nutritional Considerations

  • Dietary recommendations include foods rich in fruits, grains, and cereals.
  • Keep consumption of processed and refined foods to a minimum.
  • Consider that taking a multivitamin containing B12 and folate may be recommended for patients with elevated homocysteine levels related to a dietary deficiency.
  • Instruct the folate-deficient patient (especially pregnant women), as appropriate, to eat foods rich in folate, such as liver, salmon, eggs, asparagus, green leafy vegetables, broccoli, sweet potatoes, beans, and whole wheat.
  • Instruct the patient with vitamin B12 deficiency, as appropriate, in the use of vitamin supplements. Inform the patient, as appropriate, that the best dietary sources of vitamin B12 are meats, fish, poultry, eggs, and milk.

Clinical Judgement

  • Consider who can provide the best support to facilitate lifestyle changes and improve health.

Follow-Up and Desired Outcomes