Intravitreal Cefazolin (2.25 mg/0.1 mL)

Reconstitute a 500-mg vial of cefazolin with 2 mL of sterile water. Draw 1 mL of the solution into a Tb syringe and inject into an empty 30-mL vial. Add 9 mL of sterile water. Mix. Withdraw 0.2 mL of the solution from the 30-mL vial into a Tb syringe. Remove the Tb syringe needle and replace it with a 30-gauge needle. Expel 0.1 mL to leave 0.1 mL of 2.25-mg/0.1-mL cefazolin solution.

Intravitreal Vancomycin (1 mg/0.1 mL)

Reconstitute a 500-mg vial of vancomycin with 10 mL of sterile water. Withdraw 1 mL of the solution and inject it into a sterile 10-mL vial. Add 4 mL of sterile water to the 10-mL vial. Mix. Withdraw 0.2 mL of vancomycin solution with a Tb syringe. Remove the Tb syringe needle and replace it with a 30-gauge needle. Expel 0.1 mL to leave 0.1 mL of 1-mg/0.1-mL vancomycin solution.

Intravitreal Amikacin (400 mcg/0.1 mL)

Withdraw 0.8 mL (40 mg) of amikacin from a 100-mg/2-mL amikacin vial. Inject into a sterile 10-mL vial. Add 9.2 mL of nonpreserved sodium chloride and mix. Withdraw 0.3 mL into a sterile Tb syringe and replace the needle with a 30-gauge needle. Expel 0.2 mL to leave 0.1 mL of 400-mcg/0.1-mL amikacin solution.

Intravitreal Ceftazidime (2 mg/0.1 mL)

Add 9.4 mL of sterile water to 1 g of ceftazidime injection (in a vial). After dissolving, vent the vial. From the ceftazidime vial, transfer 2 mL to a 10-mL sterile vial. To this sterile vial, add 8 mL of sodium chloride 0.9% nonpreserved. Withdraw 0.3 mL of the solution into a sterile Tb syringe and replace the needle with a 30-gauge needle. Expel 0.2 mL to leave 0.1 mL of a 2-mg/0.1-mL ceftazidime solution.