Hyaluronidase is derived from bovine (Amphadase, Amphastar) or ovine (Vitrase, Bausch & Lomb) testicular hyaluronidase.4059; 4060
Hyaluronidase (Amphadase) is available as a 150-unit/mL solution in 1-mL single-dose vials.4059 Each mL contains hyaluronidase 150 units with calcium chloride 0.4 mg, edetate disodium 1 mg, monobasic sodium phosphate 1.7 mg, sodium chloride 8.5 mg, and thimerosal not more than 0.1 mg in water for injection.4059 Sodium hydroxide may have been added for pH adjustment.4059
Hyaluronidase (Vitrase) is available as a 200-unit/mL solution in 1-mL single-dose vials.4060 Each mL contains hyaluronidase 200 units, dibasic potassium phosphate 0.36 mg, lactose monohydrate 0.93 mg, monobasic potassium phosphate 0.23 mg, and sodium chloride 9 mg.4060
pH
Amphadase: 6.8.4059
Vitrase: 6.4 to 7.2.4060
Osmolality
Amphadase: 295 to 355 mOsm/kg.4059
Trade Name(s)
Amphadase, Vitrase
Hyaluronidase, depending on the specific product, may be administered by infiltration, or by subcutaneous, intramuscular, interstitial, intraocular, peribulbar, retrobulbar, or soft tissue injection.4059; 4060 Hyaluronidase should not be administered intravenously because the enzyme is rapidly inactivated.4059; 4060
Hyaluronidase is a clear, colorless solution.4059; 4060 Intact vials should be stored at 2 to 8°C.4059; 4060 Product labeling for Vitrase also states to protect from light and do not freeze.4060
Hyaluronidase is stated to be incompatible with benzodiazepines.4059; 4060
Hyaluronidase is stated to be incompatible with products containing sodium metabisulfite (e.g., lidocaine 2% with 1:100,000 or 1:200,000 epinephrine).4059
Hyaluronidase (Wyeth) 75 units/mL in citric acid/sodium citrate buffer (pH 4.5) was found to lose about 7 to 8% activity in 24 hours at 4 and 23°C. Hyaluronidase activity decreased by 25 to 33% in 48 hours.1907
For a list of references cited in the text of this monograph, search the monograph titled References.