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Useful for determining presence of herpes viruses (herpes simplex virus or herpes zoster virus). Optimal lesion to sample is an early vesicle. Lesion is gently unroofed with no. 15 scalpel blade, and base of vesicle is gently scraped with belly of blade (keep blade perpendicular to skin surface to prevent laceration). Scrapings are transferred to slide and stained with Wright's or Giemsa stain. A positive preparation has multinucleated giant cells. Culture or immunofluorescence testing must be performed to identify the specific virus.

Outline

Section 5. Dermatology