Diagnostic evaluation of CSF is based on the clinical scenario. In general, spinal fluid should always be sent for cell count with differential, protein, and glucose. Other studies that can be obtained on CSF include bacterial mycobacterial fungal, viral cultures, polymerase chain reaction (PCR) tests for microorganisms, smears (Gram and acid-fast stains), VDRL, cryptococcal antigen, gamma globulins, oligoclonal bands, and cytology.